Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.05
2',3'-didehydro-2',3'-dideoxythymidine
-
at pH 7.8 and 25°C
0.075
2',3'-dideoxy-2',3'-didehydrothymidine
37°C, pH not specified in the publication
0.00065 - 0.0087
2',3'-dideoxy-3'-azidothymidine
0.03
2',3'-dideoxythymidine
37°C, pH not specified in the publication
0.004 - 0.025
2'-deoxycytidine
0.0011 - 0.33
2'-deoxythymidine
0.0015 - 0.011
2'-deoxyuridine
0.132
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
37°C, pH not specified in the publication
0.032
3'-azido-2',3'-dideoxythymidine
-
at pH 7.8 and 25°C
0.00038 - 2.318
3'-azido-3'-deoxythymidine
0.0563
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0174
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0318
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0356
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0552
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0799
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0373
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0552
3-[1(2)-propyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.002
5-bromo-2'-deoxyuridine
pH 7.4, 25°C
0.001 - 0.04
5-fluoro-2'-deoxyuridine
0.03
5-fluorodeoxyuridine
-
full-length enzyme, at pH 7.8 and 37°C
0.0967
5-hydroxymethyl-2'-deoxyuridine
pH 7.4, 25°C
0.0012
5-iodo-2'-deoxyuridine
-
-
0.011
aciclovir monophosphate
Herpes simplex virus
-
-
0.00337 - 0.417
acyclovir
0.29
beta-L-thymidine
-
at pH 7.8 and 25°C
8.4
deoxyadenosine
-
full-length enzyme, at pH 7.8 and 37°C
0.003 - 0.568
deoxycytidine
2.6
deoxyguanosine
-
full-length enzyme, at pH 7.8 and 37°C
2.5
deoxyinosine
-
full-length enzyme, at pH 7.8 and 37°C
0.0017
deoxythymidine
-
-
0.0047 - 0.43
deoxyuridine
0.066
difluorodeoxycytidine
-
0.029
difluorodeoxyuridine
-
0.114
dioxolane thymidine
37°C, pH not specified in the publication
0.00333 - 0.473
ganciclovir
0.12
ganciclovir monophosphate
Herpes simplex virus
-
-
0.022
trifluoromethylthymidine
-
at pH 7.8 and 25°C
additional information
additional information
-
0.00065
2',3'-dideoxy-3'-azidothymidine
37°C, pH not specified in the publication
0.0087
2',3'-dideoxy-3'-azidothymidine
82°C, pH not specified in the publication
0.004
2'-deoxycytidine
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C
0.0049
2'-deoxycytidine
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C
0.018
2'-deoxycytidine
tetrameric enzyme, pH 7.5, 25°C
0.025
2'-deoxycytidine
dimeric enzyme, pH 7.5, 25°C
0.0011
2'-deoxythymidine
pH 7.4, 25°C
0.0058
2'-deoxythymidine
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C
0.0065
2'-deoxythymidine
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C
0.016
2'-deoxythymidine
-
37°C, pH 7.6
0.025
2'-deoxythymidine
-
pH 8.0, 37°C
0.041
2'-deoxythymidine
-
37°C, pH 7.6
0.05
2'-deoxythymidine
tetrameric enzyme, pH 7.5, 25°C
0.054
2'-deoxythymidine
dimeric enzyme, pH 7.5, 25°C
0.33
2'-deoxythymidine
-
37°C, pH 7.6
0.0015
2'-deoxyuridine
37°C, pH not specified in the publication
0.011
2'-deoxyuridine
pH 7.4, 25°C
0.00038
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.00043
3'-azido-3'-deoxythymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.00052
3'-azido-3'-deoxythymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0027
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.003
3'-azido-3'-deoxythymidine
pH 7.4, 25°C
0.012
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.015
3'-azido-3'-deoxythymidine
-
37°C, pH 7.6
0.018
3'-azido-3'-deoxythymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.033
3'-azido-3'-deoxythymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.05
3'-azido-3'-deoxythymidine
-
37°C, pH 7.6
0.204
3'-azido-3'-deoxythymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.254
3'-azido-3'-deoxythymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.406
3'-azido-3'-deoxythymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.6
3'-azido-3'-deoxythymidine
-
37°C, pH 7.6
2.318
3'-azido-3'-deoxythymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.001
5-fluoro-2'-deoxyuridine
37°C, pH not specified in the publication
0.0057
5-fluoro-2'-deoxyuridine
pH 7.4, 25°C
0.04
5-fluoro-2'-deoxyuridine
-
at pH 7.8 and 25°C
0.00337
acyclovir
Herpes simplex virus
-
37°C, mutant SR26
0.0056
acyclovir
Herpes simplex virus
-
37°C, mutant SR11
0.00979
acyclovir
Herpes simplex virus
-
37°C, mutant SR39
0.417
acyclovir
Herpes simplex virus
-
37°C, wild-type
0.0001
ATP
-
two active sites for ATP
0.0001
ATP
-
ATP in form of MgATP2-
0.0013
ATP
Herpes simplex virus
-
pH 7.6, 37°C, mutant C336Y
0.0028
ATP
-
dNTP-free recombinant dimeric enzyme, pH 7.5, 25°C
0.0028
ATP
-
ATP in form of MgATP2-
0.0047
ATP
dimeric enzyme, pH 7.5, 25°C
0.0059
ATP
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C
0.0059
ATP
-
ATP in form of MgATP2-
0.0109
ATP
tetrameric enzyme, pH 7.5, 25°C
0.013
ATP
Herpes simplex virus
-
pH 7.6, with deoxycytidine
0.021
ATP
Herpes simplex virus
-
pH 7.6, with deoxyuridine
0.025
ATP
wild-type, thymidine as substrate, pH 7.6
0.03
ATP
Herpes simplex virus
-
pH 7.6, 37°C, wild-type
0.03
ATP
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.037
ATP
wild-type, deoxycytidine as substrate, pH 7.6
0.04
ATP
37°C, pH not specified in the publication
0.041
ATP
-
recombinant mutant T298S, pH 7.5, 37°C
0.049
ATP
-
recombinant mutant G294A, pH 7.5, 37°C
0.058
ATP
K0.5 value, Hill coefficient 1.6, pH 7.4, 25°C
0.059
ATP
I212 mutant, deoxycytidine as substrate, pH 7.6
0.07
ATP
-
cytosolic enzyme, pH 7.5, 37°C
0.076
ATP
-
pH 8, 25°C or 37°C
0.08
ATP
H121N mutant, thymidine as substrate, pH 7.6
0.088
ATP
-
recombinant mutant K297R, pH 7.5, 37°C
0.095
ATP
I212 mutant, thymidine as substrate, pH 7.6
0.098
ATP
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.1
ATP
-
mitochondrial enzyme, pH 7.5, 37°C
0.11
ATP
-
mitochondrial enzyme, pH 7.5, 37°C
0.118
ATP
Herpes simplex virus
-
-
0.14
ATP
-
at pH 7.8 and 25°C
0.162
ATP
H121N mutant, deoxycytidine as substrate, pH 7.6
0.18
ATP
-
full-length enzyme, with 0.0005 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.187
ATP
82°C, pH not specified in the publication
0.21
ATP
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.22
ATP
-
full-length enzyme, with 0.005 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.32
ATP
-
full-length enzyme, with 0.5 mM deoxyinosine as cosubstrate, at pH 7.8 and 37°C
0.9
ATP
-
two active sites for ATP
0.9
ATP
-
ATP in form of MgATP2-
2.6
ATP
-
at concentrations above 1 mM, pH 8, 37°C
20
ATP
-
at concentrations below 1 mM, pH 8, 37°C
0.049
CTP
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.057
CTP
-
about, recombinant mutant T298S, pH 7.5, 37°C
0.088
CTP
-
recombinant mutant G294A, pH 7.5, 37°C
0.108
CTP
-
recombinant mutant K297R, pH 7.5, 37°C
0.003
deoxycytidine
-
mitochondrial enzyme, pH 7.5, 37°C
0.0036
deoxycytidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.006
deoxycytidine
-
pH 7.4, 37°C
0.011
deoxycytidine
wild-type and H121N mutant, pH 7.6
0.0115
deoxycytidine
mTK2-55 pH 7.6
0.0236
deoxycytidine
mTK2-39 pH 7.6
0.0251
deoxycytidine
mTK2 pH 7.6
0.0414
deoxycytidine
-
pH and temperature not specified in the publication
0.106
deoxycytidine
Herpes simplex virus
-
pH 7.6
0.568
deoxycytidine
I212 mutant, pH 7.6
0.0047
deoxyuridine
Herpes simplex virus
-
pH 7.6
0.0066
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.015
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.08
deoxyuridine
-
full-length enzyme, at pH 7.8 and 37°C
0.118
deoxyuridine
-
at pH 7.8 and 25°C
0.43
deoxyuridine
-
thymidine kinase domain 2, at pH 7.8 and 37°C
0.00333
ganciclovir
Herpes simplex virus
-
37°C, mutant SR39
0.00614
ganciclovir
Herpes simplex virus
-
37°C, mutant SR11
0.01755
ganciclovir
Herpes simplex virus
-
37°C, mutant SR26
0.0476
ganciclovir
Herpes simplex virus
-
37°C, wild-type
0.05
ganciclovir
Herpes simplex virus
-
pH 7.6, 37°C, mutant Q125E
0.069
ganciclovir
Herpes simplex virus
-
pH 7.6, 37°C, wild-type
0.473
ganciclovir
Herpes simplex virus
-
pH 7.6, 37°C, mutant Q125N
0.041
GTP
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.043
GTP
-
about, recombinant mutant K297R, pH 7.5, 37°C
0.045
GTP
-
recombinant mutant T298S, pH 7.5, 37°C
0.069
GTP
-
recombinant mutant G294A, pH 7.5, 37°C
0.00015
thymidine
-
pH 8.5, 30°C
0.0002
thymidine
Herpes simplex virus
-
pH 7.2, wild-type
0.00023
thymidine
P03176
mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.00027
thymidine
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0003
thymidine
-
pH 7.5, recombinant mutant V106H, in absence of ATP
0.0003
thymidine
-
pH 7.5, recombinant mutants V106A and V106G, in presence of ATP
0.0003
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00038
thymidine
Herpes simplex virus
-
37°C, wild-type
0.0004
thymidine
-
pH 7.5, recombinant mutants V106L and V106G, in absence of ATP
0.0004
thymidine
-
pH 7.5, recombinant mutants V106L, V106H and V106T, in presence of ATP
0.00044
thymidine
P03176
wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0005
thymidine
37°C, pH not specified in the publication
0.0005
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00054
thymidine
-
pH 8.0, 37°C, recombinant wild-type enzyme
0.0006
thymidine
Herpes simplex virus
-
-
0.0006
thymidine
-
pH 7.5, recombinant mutant V106M, in absence or presence of ATP
0.0006
thymidine
-
pH 7.5, recombinant wild-type enzyme, in presence of ATP
0.0006
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00062
thymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.0007
thymidine
-
TK-A, below 0.004 mM deoxythymidine, 25°C
0.0007
thymidine
-
pH 7.5, recombinant mutant V106K, in absence of ATP
0.0007
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0007
thymidine
subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0008
thymidine
-
pH 7.5, recombinant mutant V106Q, in absence or presence of ATP
0.0009
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, wild-type
0.0009
thymidine
-
pH 7.5, recombinant mutant V106I, in presence of ATP
0.0009
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0009
thymidine
subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0009
thymidine
subunit beta, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00095
thymidine
Herpes simplex virus
-
37°C, mutant SR11
0.001
thymidine
-
TK-C, below 0.004 mM deoxythymidine, 25°C
0.001
thymidine
82°C, pH not specified in the publication
0.0012
thymidine
-
pH 7.5, recombinant mutant V106K, in presence of ATP
0.0012
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0013
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, wild-type
0.0013
thymidine
Herpes simplex virus
-
37°C, mutant SR26
0.0013
thymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0014
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0014
thymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.0014
thymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0016
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.002
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0022
thymidine
-
pH 8, 37°C
0.0023
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0024
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0025
thymidine
Herpes simplex virus
-
pH 7.2, Y101F mutant
0.0025
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0026
thymidine
-
cytoplasmic enzyme, pH 7.5, 37°C
0.0026
thymidine
-
mitochondrial enzyme, pH 7.5, 37°C
0.00264
thymidine
Herpes simplex virus
-
37°C, mutant SR39
0.003
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, mutant Q125N
0.0034
thymidine
mTK2 pH 7.6
0.0039
thymidine
P03176
mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0039
thymidine
-
thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0041
thymidine
-
pH 8, 25°C or 37°C
0.0043
thymidine
-
wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0049
thymidine
mTK2-39 pH 7.6
0.0049
thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.005
thymidine
-
pH 7.4, 37°C
0.005 - 0.0052
thymidine
-
mitochondrial enzyme, pH 7.5, 37°C
0.0051
thymidine
-
thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0056
thymidine
-
pH 8.0, 37°C, Km is markedly dependent on pH
0.0056
thymidine
-
thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0057
thymidine
H121N mutant, pH 7.6
0.00596
thymidine
-
pH 8.0, 37°C, recombinant mutant S13D
0.006
thymidine
-
thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.007
thymidine
-
TK-A, above 0.004 mM deoxythymidine, 25°C
0.0073
thymidine
mTK2-55 pH 7.6
0.0078
thymidine
-
full-length enzyme, at pH 7.8 and 37°C
0.008
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.009
thymidine
-
pH 7.8, 37°C
0.01
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, mutant C336Y
0.01
thymidine
-
cytosolic enzyme, pH 7.5, 37°C
0.01
thymidine
-
TK-C, above 0.004 mM deoxythymidine, 25°C
0.012
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0127
thymidine
-
pH 7.5, recombinant mutant V106A, in absence of ATP
0.013
thymidine
wild-type, pH 7.6
0.016
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.018
thymidine
I212 mutant, pH 7.6
0.0188
thymidine
-
pH and temperature not specified in the publication
0.02
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, mutant Q125E
0.0271
thymidine
-
pH 7.5, recombinant wild-type enzyme, in absence of ATP
0.0294
thymidine
-
pH 7.5, recombinant mutant V106I, in absence of ATP
0.036
thymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.037
thymidine
P03176
mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.043
thymidine
-
pH 7.5, recombinant mutant V106T, in absence of ATP
0.046
thymidine
-
thymidine kinase domain 2, at pH 7.8 and 37°C
0.048
thymidine
-
at pH 7.8 and 25°C
0.057
thymidine
-
pH 7, 37°C
0.059
thymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.074
thymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.263
thymidine
P03176
mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.317
thymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.336
thymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.349
thymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.565
thymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.59
thymidine
P03176
mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.612
thymidine
P03176
mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
6
thymidine
Herpes simplex virus
-
pH 7.6, 37°C, mutant Q125D
0.072
TTP
-
recombinant wild-type enzyme, pH 7.5, 37°C
0.127
TTP
-
recombinant mutant T298S, pH 7.5, 37°C
1.462
uridine
pH 7.4, 25°C
7.8
uridine
-
full-length enzyme, at pH 7.8 and 37°C
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
Tequatrovirus T4
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
additional information
additional information
-
-
-
additional information
additional information
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics of wild-type and mutant enzymes in absence or presence of ATP, overview
-
additional information
additional information
-
kinetics, ATP-binding kinetics of wild-type and mutant enzymes at room temperature
-
additional information
additional information
-
steady-state kinetics, kinetics of conformational changes induced by ligand binding
-