2.7.1.21: thymidine kinase
This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.
Word Map on EC 2.7.1.21
-
2.7.1.21
-
herpes
-
simplex
-
ganciclovir
-
suicide
-
nucleoside
-
hsv-tk
-
acyclovir
-
viruses
-
triphosphate
-
adenovirus
-
prodrugs
-
salvage
-
lymphocyte
-
vaccinia
-
herpesvirus
-
pyrimidine
-
bystander
-
retroviral
-
chloramphenicol
-
positron
-
glioma
-
intratumoral
-
cotransfection
-
deoxycytidine
-
varicella-zoster
-
cytomegalovirus
-
tomography
-
nude
-
lymphoma
-
dna-mediated
-
dtmp
-
deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
-
deoxyguanosine
-
deoxyribonucleoside
-
synthesis
-
medicine
-
herpetic
-
diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
-
zidovudine
-
kinase-deficient
-
molecular biology
-
3'-azido-3'-deoxythymidine
-
pseudorabies
-
replication-competent
-
varicella
-
zoster
-
5-fluorocytosine
- 2.7.1.21
- herpes
- simplex
- ganciclovir
-
suicide
- nucleoside
-
hsv-tk
- acyclovir
- viruses
- triphosphate
- adenovirus
-
prodrugs
-
salvage
- lymphocyte
- vaccinia
- herpesvirus
- pyrimidine
-
bystander
-
retroviral
- chloramphenicol
-
positron
- glioma
-
intratumoral
-
cotransfection
- deoxycytidine
-
varicella-zoster
- cytomegalovirus
-
tomography
-
nude
- lymphoma
-
dna-mediated
- dtmp
- deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
- deoxyguanosine
- deoxyribonucleoside
- synthesis
- medicine
-
herpetic
- diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
- zidovudine
-
kinase-deficient
- molecular biology
- 3'-azido-3'-deoxythymidine
- pseudorabies
-
replication-competent
- varicella
- zoster
- 5-fluorocytosine
Reaction
Synonyms
2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK
ECTree
2.7.1.21 thymidine kinaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.7.1.21 - thymidine kinase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
ATP + 1-(beta-D-arabinofuranosyl)thymine
ADP + 1-(beta-D-arabinofuranosyl)thymine 5'-phosphate
ATP + 1-beta-D-arabinofuranosylthymine
ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
as active as with 2'-deoxythymidine
-
-
?
ATP + 2',3'-didehydro-2',3'-dideoxythymidine
ADP + 2',3'-didehydro-2',3'-dideoxythymidine 5'-phosphate
-
12% activity compared to thymidine
-
-
?
ATP + 2',3'-dideoxy-2',3'-didehydrothymidine
ADP + 2',3'-dideoxy-2',3'-didehydrothymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxy-3'-azidothymidine
ADP + 2',3'-dideoxy-3'-azidothymidine 5'-phosphate
-
-
-
?
ATP + 2'-exo-methanocarba-thymidine
ADP + 2'-exo-methanocarba-thymidine 5'-monophosphate
Herpes simplex virus
-
MCT
-
?
ATP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
ADP + 2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil 5'-phosphate
-
-
-
?
ATP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
ATP + 3'-azido-2',3'-deoxythymidine
ADP + 3'-azido-2',3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-deoxy-2',3'-didehydro-thymidine
ADP + 3'-deoxy-2',3'-didehydro-thymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-deoxy-2',3'-didehydrothymidine
ADP + 3'-deoxy-2',3'-didehydrothymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-deoxythymidine
ADP + 3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-fluoro-3'-deoxythymidine
ADP + 3'-fluoro-3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3-(benzylcarbamimidoyl)thymidine
ADP + 3-(benzylcarbamimidoyl)thymidine 5'-phosphate
-
14.9% activity compared to thymidine
-
-
?
ATP + 3-(butylcarbamimidoyl)thymidine
ADP + 3-(butylcarbamimidoyl)thymidine 5'-phosphate
-
1.2% activity compared to thymidine
-
-
?
ATP + 3-(phenylcarbamimidoyl)thymidine
ADP + 3-(phenylcarbamimidoyl)thymidine 5'-phosphate
-
1.9% activity compared to thymidine
-
-
?
ATP + 3-(tetrazol-5-ylmethyl)thymidine
ADP + 3-(tetrazol-5-ylmethyl)thymidine 5'-phosphate
-
39.5% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine 5'-phosphate
-
63.7% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine 5'-phosphate
-
42.5% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine 5'-phosphate
-
52.4% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine 5'-phosphate
-
55.6% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine 5'-phosphate
-
58.2% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine 5'-phosphate
-
42.9% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
ADP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine 5'-phosphate
-
55.7% activity compared to thymidine
-
-
?
ATP + 3-[1(2)-propyltetrazol-5-yl]thymidine
ADP + 3-[1(2)-propyltetrazol-5-yl]thymidine 5'-phosphate
-
57.6% activity compared to thymidine
-
-
?
ATP + 3-[2-(closo-1,7-carboranyl)ethylcarbamimidoyl]thymidine
ADP + 3-[2-(closo-1,7-carboranyl)ethylcarbamimidoyl]thymidine 5'-phosphate
-
16.3% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-(benzylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(benzylamino)ethyl]thymidine 5'-phosphate
-
16.8% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-(butylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(butylamino)ethyl]thymidine 5'-phosphate
-
2.3% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-(phenylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(phenylamino)ethyl]thymidine 5'-phosphate
-
11.3% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-(propylamino)ethyl]thymidine
ADP + 3-[2-imino-2-(propylamino)ethyl]thymidine 5'-phosphate
-
1.9% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)ethylamino]ethyl]thymidine
ADP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)ethylamino]ethyl]thymidine 5'-phosphate
-
3.0% activity compared to thymidine
-
-
?
ATP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)propylamino]ethyl]thymidine
ADP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)propylamino]ethyl]thymidine 5'-phosphate
-
5.6% activity compared to thymidine
-
-
?
ATP + 3-[3-(closo-1,7-carboranyl)propylcarbamimidoyl]thymidine
ADP + 3-[3-(closo-1,7-carboranyl)propylcarbamimidoyl]thymidine 5'-phosphate
-
15.5% activity compared to thymidine
-
-
?
ATP + 3-[4-(closo-1,7-carboranyl)butylcarbamimidoyl]thymidine
ADP + 3-[4-(closo-1,7-carboranyl)butylcarbamimidoyl]thymidine 5'-phosphate
-
27.3% activity compared to thymidine
-
-
?
ATP + 5-azido-3'-azido-2',3'-dideoxyuridine
ADP + 5-azido-3'-azido-2',3'-dideoxyuridine 5'-monophosphate
Herpes simplex virus
-
-
photoaffinity analog of 3'-azidothymidine
?
ATP + 5-bromodeoxyuridine
ADP + 5-bromodeoxyuridine 5'-phosphate
-
84% activity compared to thymidine
-
-
?
ATP + 5-chlorodeoxyuridine
ADP + 5-chlorodeoxyuridine 5'-phosphate
-
110% activity compared to thymidine
-
-
?
ATP + 5-diaza-2'-deoxyuridine
ADP + 5-diaza-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-fluorodeoxyuridine
ADP + 5-fluorodeoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-hydroxymethyl-2'-deoxyuridine
ADP + 5-hydroxymethyl-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
?
5-iodo-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + 5-iododeoxyuridine
ADP + 5-iododeoxyuridine 5'-phosphate
-
89% activity compared to thymidine
-
-
?
ATP + 5-mercapto-2'-deoxyuridine
ADP + 5-mercapto-2'-deoxyuridine 5'-phosphate
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
-
?
ATP + 5-trifluoromethyl-2'-deoxyuridine
ADP + 5-trifluoromethyl-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-trifluoromethyl-2-deoxyuridine
?
5-trifluoromethyl-2-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + 6-aza-2'-deoxythymidine
ADP + 6-aza-2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine
ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-phosphate
-
-
-
-
?
ATP + 9-[(hydroxyethoxy)methyl]guanine
ADP + 9-[(hydroxyethoxy)methyl]guanine 5'-phosphate
-
-
-
-
?
ATP + beta-5-ethyl-2'-deoxyuridine
?
beta-5-ethyl-2'-deoxyuridine is efficiently phosphorylated by thymidine kinase confirming that these compound enters the active site of the enzyme and acts as alternate substrate
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
ATP + beta-L-thymidine
ADP + beta-L-thymidine 5'-phosphate
-
less than 5% activity compared to thymidine
-
-
?
ATP + deoxythymidine
ADP + dTMP
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
?
ATP + dioxolane thymidine
ADP + dioxolane thymidine 5'-phosphate
-
-
-
?
ATP + iododeoxyuridine
ADP + iododeoxyuridine 5'-phosphate
-
-
-
-
?
ATP + thymidine 5'-phosphate
ADP + thymidine diphosphate
Herpes simplex virus type 4
-
-
-
-
r
ATP + trifluoromethylthymidine
ADP + trifluoromethylthymidine 5’-phosphate
-
good substrate, 130% activity compared to thymidine
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
about 115% of the activity with 2'-deoxythymidine
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + (E)-5-(2-bromovinyl)-2'-deoxyuridine
ADP + (E)-5-(2-bromovinyl)-2'-deoxyuridine 5'-phosphate
-
drug activation by phosphorylation
-
-
?
ATP + 1-(beta-D-arabinofuranosyl)thymine
ADP + 1-(beta-D-arabinofuranosyl)thymine 5'-phosphate
-
-
-
?
ATP + 1-(beta-D-arabinofuranosyl)thymine
ADP + 1-(beta-D-arabinofuranosyl)thymine 5'-phosphate
-
-
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 2',3'-dideoxycytidine
ADP + 2',3'-dideoxycytidine 5'-phosphate
-
-
-
-
?
ATP + 2',3'-dideoxydehydrothymidine
ADP + 2',3'-dideoxydehydrothymidine 5'-phosphate
-
-
-
-
?
ATP + 2',3'-dideoxydehydrothymidine
ADP + 2',3'-dideoxydehydrothymidine 5'-phosphate
-
-
-
-
?
ATP + 2',3'-dideoxythymidine
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2',3'-dideoxythymidine
ADP + 2',3'-dideoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxycytidine
ADP + 2'-deoxycytidine 5'-phosphate
-
induction of conformational changes upon ligand binding, overview
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
induction of conformational changes upon ligand binding, overview
-
-
ir
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 2'-deoxythymidine
ADP + 2'-deoxythymidine 5'-phosphate
-
-
-
-
ir
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 2'-deoxyuridine
ADP + 2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
2.4% activity compared to thymidine
-
-
?
ATP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-phenylethyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
12.3% activity compared to thymidine
-
-
?
ATP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
3.1% activity compared to thymidine
-
-
?
ATP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(2-pyridine)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
7.1% activity compared to thymidine
-
-
?
ATP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
2.7% activity compared to thymidine
-
-
?
ATP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(3-chloropropyl)-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
6.5% activity compared to thymidine
-
-
?
ATP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
2.6% activity compared to thymidine
-
-
?
ATP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine
ADP + 3'-(4-(4-fluorophenyl)-1,2,3-triazole-1-yl)-3'-deoxythymidine 5'-phosphate
13.7% activity compared to thymidine
-
-
?
ATP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
4.8% activity compared to thymidine
-
-
?
ATP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-benzyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
11.6% activity compared to thymidine
-
-
?
ATP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
2.9% activity compared to thymidine
-
-
?
ATP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-pentyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
8.7% activity compared to thymidine
-
-
?
ATP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
2.1% activity compared to thymidine
-
-
?
ATP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-phenyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
5.5% activity compared to thymidine
-
-
?
ATP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
1.9% activity compared to thymidine
-
-
?
ATP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-propyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
7.2% activity compared to thymidine
-
-
?
ATP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
2.2% activity compared to thymidine
-
-
?
ATP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine
ADP + 3'-(4-tert-butyl-1,2,3-triazol-1-yl)-3'-deoxythymidine 5'-phosphate
4.7% activity compared to thymidine
-
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
about 5% of the activity with 2'-deoxythymidine
-
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
60% activity compared to thymidine
-
-
?
ATP + 3'-azido-2',3'-dideoxythymidine
ADP + 3'-azido-2',3'-dideoxythymidine 5'-phosphate
-
about 165% of the activity with 2'-deoxythymidine
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
Q8LDP6, Q9FN47
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
47.3% activity compared to thymidine
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
-
-
-
?
ATP + 3'-azido-3'-deoxythymidine
ADP + 3'-azido-3'-deoxythymidine 5'-phosphate
27.8% activity compared to thymidine
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-bromo-2'-deoxyuridine
ADP + 5-bromo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
-
?
ATP + 5-chloro-2'-deoxyuridine
ADP + 5-chloro-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
good substrate, 120% activity compared to thymidine
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-fluoro-2'-deoxyuridine
ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + 5-iodo-2'-deoxyuridine
ADP + 5-iodo-2'-deoxyuridine 5'-phosphate
-
-
-
-
?
ATP + acyclovir
ADP + acyclovir phosphate
-
drug activation by phosphorylation
-
-
?
ATP + acyclovir
ADP + acyclovir phosphate
-
drug activation by phosphorylation, some naturally occurring mutations lead to inability of acyclovir phosphorylation and thus resistance against the drug
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
-
?
ATP + beta-L-2',3'-dideoxy-3'-thiacytidine
ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate
-
-
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
substrate for mutant enzymes M42I and L134A
-
-
?
ATP + deoxycytidine
ADP + dCMP
-
7% activity compared to thymidine
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
substrate for mutant enzymes S182T and S182A
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
about 20% activity compared to thymidine
-
-
?
ATP + deoxyguanosine
ADP + dGMP
-
about 20% activity compared to thymidine
-
-
?
ATP + deoxyinosine
ADP + dIMP
-
about 30% activity compared to thymidine
-
-
?
ATP + deoxyinosine
ADP + dIMP
-
about 30% activity compared to thymidine
-
-
?
ATP + deoxyuridine
ADP + dUMP
-
69% activity compared to thymidine
-
-
?
ATP + deoxyuridine
ADP + dUMP
-
ATP as phosphate donor can be replaced by dGTP, dATP, dCTP, GTP, and ITP
-
?
ATP + deoxyuridine
ADP + dUMP
-
about 185% activity compared to thymidine
-
-
?
ATP + thymidine
ADP + dTMP
Herpes simplex virus
-
-
-
-
?
ATP + thymidine
ADP + dTMP
-
-
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by GTP
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by dGTP
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by UTP
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by dCTP
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by dATP
-
-
?
ATP + thymidine
ADP + dTMP
-
ATP can be substituted by CTP
-
-
?
ATP + thymidine
ADP + dTMP
-
best substrate combination, 100% activity
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
ATP acts as specific phosphate donor
-
-
?
ATP + thymidine
ADP + thymidine 5'-phosphate
-
enzyme contains the putative thymidine-binding motif FQRK
-
-
?
additional information
?
-
-
the wild type enzyme shows no activity with deoxycytidine and deoxyadenosine
-
-
?
additional information
?
-
-
the type II thymidine kinase ecoded by cowpox virus exhibits a more limited substrate specificity than the type I thymidine kinase encoded by HSV-1
-
-
?
additional information
?
-
-
CTP, diphosphate, uridine, adenosine, cytosine, deoxyguanosine, deoxyguanosine, dTMP, thymine 1-beta-D-arabinofuranoside, 1-beta-D-arabinofuranosylcytosine, acyclovir, and 5-iodo-2'-deoxycytidine are no substrates
-
-
?
additional information
?
-
beta-L-thymidine is poorly phosphorylated by the enzyme
-
-
?
additional information
?
-
-
beta-L-thymidine is poorly phosphorylated by the enzyme
-
-
?
additional information
?
-
does not use UTP, GTP, and TTP as cosubstrates
-
-
?
additional information
?
-
-
does not use UTP, GTP, and TTP as cosubstrates
-
-
?
additional information
?
-
Herpes simplex virus type 4
-
wide substrate specificity phosphorylating pyrimidine nucleosides, nucleoside monophophates, and several nucleoside analogues
-
-
?
additional information
?
-
the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs
-
-
?
additional information
?
-
-
the cell-cycle-regulated enzyme is important in nucleotide metabolism and an activator of antiviral and anticancer drugs
-
-
?
additional information
?
-
-
the enzyme is tightly regulated in the cell cycle by multiple mechanism, e.g. by phosphorylation at Ser13 in mitotic-arrested cells, regulation model
-
-
?
additional information
?
-
-
ligand-induced conformational changes in dimeric and tetrameric forms of the enzyme alter the catalytic activity
-
-
?
additional information
?
-
-
Val106 has effects on structure-function relation of the enzyme
-
-
?
additional information
?
-
-
no activity with 3-(propylcarbamimidoyl)thymidine and 3-(tetrazol-5-yl)thymidine
-
-
?
additional information
?
-
-
the type II thymidine kinase ecoded by cowpox virus exhibits a more limited substrate specificity than the type I thymidine kinase encoded by HSV-1
-
-
?
additional information
?
-
-
overview thymine nucleosides, 2'-deoxyribonucleosides, arabinosides, ribonucleosides
-
-
?
additional information
?
-
-
the viral enzyme shows a broader substrate specificity than the human host cell enzyme
-
-
?
additional information
?
-
-
modeling of ganciclovir binding into the enzyme crystal structure, overview
-
-
?
additional information
?
-
-
no substrates: ganciclovir, acyclovir, 2'-deoxycytidine
-
-
?
additional information
?
-
-
substrate specificity of wild-type and mutant enzymes, overview
-
-
?
additional information
?
-
-
5-halogenated cytidines, bromouridine, iodouridine, uridine, 5-fluorodeoxycytidine, purine ribonucleosides
-
-
?
additional information
?
-
-
comparison rat liver, Trypanosoma brucei
-
-
?
additional information
?
-
Tequatrovirus T4
-
no phosphorylation of deoxyuridine, 5-halogenated deoxyuridines
-
-
?
additional information
?
-
no activity with uridine, L-thymidine or 2',3'-dideoxyuridine
-
-
?
additional information
?
-
-
no activity with uridine, L-thymidine or 2',3'-dideoxyuridine
-
-
?
additional information
?
-
no activity with uridine, L-thymidine or 2',3'-dideoxyuridine
-
-
?
additional information
?
-
-
domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine
-
-
?
additional information
?
-
-
comparison rat liver, Trypanosoma brucei
-
-
?
additional information
?
-
-
domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine
-
-
?
