1.13.11.27: 4-hydroxyphenylpyruvate dioxygenase

This is an abbreviated version!
For detailed information about 4-hydroxyphenylpyruvate dioxygenase, go to the full flat file.

Word Map on EC 1.13.11.27

Reaction

4-hydroxyphenylpyruvate
+
O2
=
homogentisate
+
CO2

Synonyms

4-HPPD, 4-hydroxyphenylpyruvare dioxygenase, 4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid dioxygenase, 4HPPD, At-HPPD, AtHPPD, AvHPPD-03, EC 1.14.2.2, EC 1.99.1.14, formerly, F Alloantigen, F protein, F-antigen homolog, HPD, hpdA, HPPD, HPPDase, Legiolysin, MsHPPD, oxygenase, 4-hydroxyphenylpyruvate di-, p-hydroxyphenyl pyruvate dioxygenase, p-hydroxyphenylpyruvate dioxygenase, p-hydroxyphenylpyruvate hydroxylase, p-hydroxyphenylpyruvate oxidase, p-hydroxyphenylpyruvic acid hydroxylase, p-hydroxyphenylpyruvic hydroxylase, p-hydroxyphenylpyruvic oxidase, PTO1369, Pt_Hpd, T-cell reactive protein, TF-AG, YS103B

ECTree

     1 Oxidoreductases
         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
             1.13.11 With incorporation of two atoms of oxygen
                1.13.11.27 4-hydroxyphenylpyruvate dioxygenase

Cloned

Cloned on EC 1.13.11.27 - 4-hydroxyphenylpyruvate dioxygenase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
barley 4-hydroxyphenylpyruvate dioxygenase gene introduced into the plastome of Nicotiana tabacum (tobacco)
DNA and amino acid sequence determination and analysis, human enzyme expression in Escherichia coli
-
expressed in Escherichia coli BL21 DE3
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expressed in Eschrichia coli stains DH5alpha, M15(pRep4) or SG13009
expressed in Glycine max (cv. Jack); expressed in Nicotiana tabacum (cv. PBD6)
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expressed in Nicotiana tabacum
-
expression in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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gene HPD, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene HPD, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
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gene HPD, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, determination of protein expression patterns
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gene MsHPPD, DNA and amio acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative reverse transcription PCR enzyme expression analysis, promoter sequence of MsHPPD is analyzed. Recombinant overexpression in Arabidopsis thaliana in primary roots, sepals, petals, stigma, filament tubes, pollens, and the ends of seeds, no expression in in the root tip or hypocotyls
overexpressed in Escherichia coli
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overexpression of Arabidopsis thaliana p-hydroxyphenylpyruvate dioxygenase and Arabidopsis thaliana homogentisate phytyltransferase in Solanum tuberosum. Over-expression of At-HPPD results in a maximum 266% increase in alpha-tocopherol, and over-expression of At-HPT yields a 106% increase. Tubers from transgenic plants accumulate approximately 10fold and 100fold less a-tocopherol than leaves or seeds, respectively
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overproduction of the recombinant enzyme in Escherichia coli JM105, overexpression of the complete coding sequence in transgenic Nicotiana tabacum using Agrobacterium tumefaciens transformation
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recombinant expression in Glycine max seeds. The AvHPPD-03 isozyme has an inherent reduced binding affinity for mesotrione, a herbicide that inhibits the wild-type soybean HPPD enzyme. Expression of avhppd-03 in soybean plants confers a mesotrione-tolerant phenotype, determination of tocopherols contents, overview
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recombinant expression of wild-type and mutant enzymes
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vaccinia virus-based expression in human AMA cells and expression in Escherichia coli
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