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Search term: diagnostics

Results 1 - 100 of 479 > >>
EC Number
Application
Commentary
aldehyde reductase
diagnostics
AKR1B10 may have a potential role as a tumor marker
shikimate dehydrogenase
diagnostics
shikimate dehydrogenase alleles can act as potential markers for flowering phenology in Pinus sylvestris. The ShDH A locus might be considered as isoenzymatic marker that differentiates these flowering groups of Scots pine clones. At several isozyme and DNA loci, the presence of private alleles in each group of pines is observed, but these alleles cannot serve as markers of Scots pine flowering time because of their low frequencies
glyoxylate reductase
diagnostics
enzyme glyoxylate reductase/hydroxypyruvate reductase is a prognostic marker for hepatocellular carcinoma patients after curative resection. Patients with negative GRHPR both in tumor tissues and nontumoral tissues have a significantly shorter survival time than those with positive GRHPR. Multivariate analysis establishes that GRHPR is detected in nontumoral tissues as an independent prognostic factor for patients with hepatocellular carcinoma, overview
L-lactate dehydrogenase
diagnostics
the enzyme is useful as marker for endometrial carcinoma
L-lactate dehydrogenase
diagnostics
low LDH affinity kinetics can be a diagnostic parameter for human breast cancer
L-lactate dehydrogenase
diagnostics
LDH levels might serve as a significant prognostic factor in high-risk patients with metastatic renal cell carcinoma (RCC) and a predictive factor associated with the response and survival benefit of the mTOR complex-1 (mTORC1) inhibitor temsirolimus. LDH is one of the risk factors included in the international prognostic index (IPI) and it is considered a strong predictor of survival of patients with aggressive lymphoid cancers. Serum LDH level inversely correlates with the survival of patients with small cell lung cancer (SCLC) and allows the selection of very high-risk patients. Serum LDH might be a useful marker for predicting global clinical outcomes in hepatocellular carcinoma patients treated with a tyrosine kinase inhibitor (sorafenib). The determination of serum LDH levels appears to be a helpful clinical tool also in the diagnosis of prostate cancer and in the control of androgenic treatment
D-lactate dehydrogenase
diagnostics
the enzyme is useful for selective determination of D,L-lactic acid in wines using peroxidase-based biosensors, method optimization, overview
3-hydroxybutyrate dehydrogenase
diagnostics
the enzyme is a useful marker in the assay of diabetes mellitus and/or ketoacidosis
3-hydroxyacyl-CoA dehydrogenase
diagnostics
the enzyme can be used as marker for early immunonological detection of infection by Leptospira in urine and of leptospirosis, a global zoonosis caused by pathogenic Leptospira
malate dehydrogenase
diagnostics
the enzyme can be used in a biosensor for malic acid detection in fruit juices, based on malate dehydrogenase (MDH), and glutamate oxaloacetate transaminase (GOT) in a powder vitreous carbon, carbon nanotubes and mineral oil, composite matrix
isocitrate dehydrogenase (NAD+)
diagnostics
enzyme NAD+-dependent isocitrate dehydrogenase cann act as a mitochondrial marker in asthenozoospermia
isocitrate dehydrogenase (NADP+)
diagnostics
IDH1 codon 132 mutation is an important prognostic biomarker in gliomas. IDH1 mutation predicts outcome in grade 2, 3, and 4 gliomas
isocitrate dehydrogenase (NADP+)
diagnostics
IDH1 mutational analysis can serve as a useful diagnostic marker of a glioma
glucose 1-dehydrogenase [NAD(P)+]
diagnostics
usage for quantitative determination of glucose in clinical tests and in the food industry
glucose 1-dehydrogenase [NAD(P)+]
diagnostics
enzyme can be used for glucose determination
17beta-estradiol 17-dehydrogenase
diagnostics
17beta-HSD1 is highly expressed in breast and ovary tissues and represents a prognostic marker for the tumor progression and survival of patients with breast cancer and other estrogen-dependent tumors
glyoxylate reductase (NADP+)
diagnostics
enzyme glyoxylate reductase/hydroxypyruvate reductase is a prognostic marker for hepatocellular carcinoma patients after curative resection. Patients with negative GRHPR both in tumor tissues and nontumoral tissues have a significantly shorter survival time than those with positive GRHPR. Multivariate analysis establishes that GRHPR is detected in nontumoral tissues as an independent prognostic factor for patients with hepatocellular carcinoma, overview
isopropanol dehydrogenase (NADP+)
diagnostics
characterization of adrenal and gonadal tissue
carnitine 3-dehydrogenase
diagnostics
the L-carnitine oxidation step can be exploited for spectroscopic L-carnitine determination in biological fluids
carbonyl reductase (NADPH)
diagnostics
expression of CBR mRNA is a significant prognostic factor in non–small-cell lung cancer and is inversely associated with tumor progression and angiogenesis
IMP dehydrogenase
diagnostics
IMPDH activity is measured in adults without thiopurine treatment as well as in adult and paediatric patients treated with thiopurines. A wide interindividual variability in IMPDH activity in erythrocytes is observed. No difference in IMPDH activity is found between untreated subjects and adult and paediatric patients on thiopurine therapy. The method described is useful in the determination of IMPDH phenotype from patients on thiopurine therapy and in the investigation of the potential relationship between IMPDH activity in erythrocytes and the occurrence of adverse events and drug response variability; IMPDH activity is measured in adults without thiopurine treatment as well as in adult and paediatric patients treated with thiopurines. A wide interindividual variability in IMPDH activity in erythrocytes is observed. No difference in IMPDH activity is found between untreated subjects and adult and paediatric patients on thiopurine therapy. The method described is useful in the determination of IMPDH phenotype from patients on thiopurine therapy and in the investigation of the potential relationship between IMPDH activity in erythrocytes and the occurrence of adverse events and drug response variability
long-chain-3-hydroxyacyl-CoA dehydrogenase
diagnostics
3-hydroxypalmitoleoyl-carnitine and other hydroxylated long chain acylcarnitines are markers of long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) and/or trifunctional protein (TFP) deficiency, while heptadecanoylcarnitine is a biomarker specific for propionic acidemia (PA) or methylmalonic acidemia (MMA) patients able to detect propionate disorders during expanded newborn screening
3alpha-hydroxysteroid 3-dehydrogenase (Re-specific)
diagnostics
co-immobilization of the enzyme with diaphorase of Clostridum sp. onto alkylamine glass beads through glutaraldehyde coupling for determination of bile acids in serum and bile in a cost-effective colorimetric assay
mannitol dehydrogenase
diagnostics
the enzyme is a major food allergen and its occurance in food, e.g. in celery sticks, celeriac, celery powder, or celery seeds, is required to be labeled, development of a quantitative PCR-based DNA detection method for use in food samples, overview
S-(hydroxymethyl)glutathione dehydrogenase
diagnostics
the enzyme is useful for determination of formaldehyde in consumers goods and environment
UDP-N-acetyl-D-mannosamine dehydrogenase
diagnostics
the wecC gene may represent a useful method for detecting the human type of Edwardsiella tarda, which may have the ability to cause human infection
glucose oxidase
diagnostics
glucose oxidase can be used in various immunoassays and/or staining procedures as well as removal of excess glucose, in real-time fluorescent microscopy for biological samples, glucose oxidase/catalase is often used for oxygen scavenging to reduce photodamage
glucose oxidase
diagnostics
used in an automatic glucose assay kit in conjunction with catalase and chiefly in biosensors for the detection and estimation of glucose in industrial solutions and in body fluids such as blood and urine
glucose oxidase
diagnostics
the enzyme is used as a molecular diagnostic and analytical tool in the medical industry for the control of diabetes
glucose oxidase
diagnostics
the enzyme is used for the manufacture of glucose biosensors and in particular sensor strips used to measure glucose levels in serum
cholesterol oxidase
diagnostics
the enzyme is used for a kinetic cholesterol assay for determination of cholesterol content in human serum, overview, the enzyme from Streptomyces is more effective than the enzyme from Brevibacterium
cholesterol oxidase
diagnostics
the enzyme is used to determine cholesterol in food and blood serum by coupling of the enzyme with peroxidase
cholesterol oxidase
diagnostics
analytic tool for determining cholesterol in various samples
galactose oxidase
diagnostics
determination of galactose or lactose concentration in complex biological fluids by immobilized galactose oxidase
(S)-2-hydroxy-acid oxidase
diagnostics
lactate biosensor development based on an enzymatic recognition system using lactate oxidase and a transduction system based on laminol, peroxidase from Arthromyces ramosus and metallic aluminum, immobilized in a plastic support by a polyion complex membrane prepared from poly-L-lysine hydrobromide and poly(sodium 4-styrenesulfonate)
(S)-2-hydroxy-acid oxidase
diagnostics
lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9
(S)-2-hydroxy-acid oxidase
diagnostics
the metabolic gene HAO2 is downregulated in hepatocellular carcinoma and predicts metastasis and poor survival. Dysregulation of HAO2 is a very early event in the development of hepatocellular carcinoma and it may represent a useful diagnostic and prognostic marker for human hepatocellular carcinoma
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
enzyme is industrially used as glucose sensor with high catalytic activity and insensitivity to oxygen
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization; the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls
glucose 1-dehydrogenase (PQQ, quinone)
diagnostics
purified enzyme is immobilized on carbon electrodes modified with 4-ferrocenylphenol, 4-(4-ferrocenylimino-methyl)-phenol, or 4-ferrocenylnitrophenol, for use as glucose biosensors, kinetic behaviour during immobilization
glucose 1-dehydrogenase (FAD, quinone)
diagnostics
glucose sensor/glucose determination
glucose 1-dehydrogenase (FAD, quinone)
diagnostics
the enzyme can be used as O2-independent biosensor for glucoe detection
choline dehydrogenase
diagnostics
study of prognostic biomarkers for breast cancer identifies the expression of CHD among three human genes controlled by estrogens, and shows that this is a strong predictor of the outcome of treatment with tamoxifen in early-stage (ER)-positive breast cancer patients
retinal dehydrogenase
diagnostics
ALDH1A1 is rapidly gaining importance as a stem cell marker
4-trimethylammoniobutyraldehyde dehydrogenase
diagnostics
detection of anti-TMABA-DH autoantibody is a potential strategy for a diagnosis of Kawasaki disease, usefulness of the anti-TMABA-DH antibody as a diagnostic marker
6-oxohexanoate dehydrogenase
diagnostics
development of a biosensor for detection of caprolactam oligomers in aqueous media
trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
diagnostics
overexpression of dihydrodiol dehydrogenase as a prognostic marker in resected gastric cancer patients, patients with DDH overexpression have a significantly higher incidence of early tumor recurrence and distant metastasis
7-dehydrocholesterol reductase
diagnostics
enzyme activity measurement can be a tool for prognosis of the Smith-Lemli-Opitz syndrome
7-dehydrocholesterol reductase
diagnostics
enzyme is a key marker of early Leydig cell steroidogenesis, using the technique of differential display RT-PCR
7-dehydrocholesterol reductase
diagnostics
enzyme activity measurement is useful for distinguishing Smith-Lemli-Opitz syndrome from carrier or unaffected cells, diagnosis of atypical cases, overview
tRNA-dihydrouridine20 synthase [NAD(P)+]
diagnostics
the enzyme defined as an independent prognostic factor for the development of non-small cell lung cancer cells
protoporphyrinogen oxidase
diagnostics
detection of the naturally occuring 1082-1083insC mutation in the PPOX gene is a genetic marker for variegate porphyria in humans
bilirubin oxidase
diagnostics
the BOD activity catalyzing the oxidation of bilirubin to biliverdin can be used for the diagnosis of jaundice and hyperbilirubinemia
bilirubin oxidase
diagnostics
BOD is used for diagnostic analysis of bilirubin in serum
succinate dehydrogenase
diagnostics
succinate dehydrogenase subunits may be candidate susceptibility genes for some phosphatase and tensin homolog (PTEN, tumor suppressor) mutation-negative individuals with Cowden syndrome-like cancers. To address this hypothesis, germline succinate dehydrogenase subunits SDHB-D mutation analysis in 375 PTEN mutation-negative Cowden syndrome /Cowden syndrome -like individuals is performed, followed by functional analysis of identified SDH mutations/variants. Germline SDH mutations/variants occur in a subset of PTEN mutation-negative Cowden syndrome /Cowden syndrome -like individuals and are associated with increased frequencies of breast, thyroid, and renal cancers beyond those conferred by germline PTEN mutations. SDH testing should be considered for germline PTEN mutation-negative Cowden syndrome /Cowden syndrome -like -like individuals, especially in the setting of breast, thyroid, and/or renal cancers
succinate dehydrogenase
diagnostics
SDHB mutations are a marker for a prognostic risk of patients with pheochromocytomas or paragangliomas
succinate dehydrogenase
diagnostics
mutations of SDH subunit encoding genes are good markers for paraganglioma
isovaleryl-CoA dehydrogenase
diagnostics
enzyme activity assay development for determination of isovaleric acidemia in newborn disorder screening
glutamate dehydrogenase
diagnostics
GLDH, gama-glutamyltransferase, aspartate-aminotransferase, alanine-aminotransferase and erythrocyte mean cell volume are assessed in 238 alcoholics admitted to hospital: on admission, after 24 h and after 7 days. All the values are significantly higher than those in healthy persons. The fastest activity decrease is seen in GLDH. The kinetics of GLDH and aspartate-aminotranferase are more applicable than gama-glutamyltransferase kinetics after a week, but GLDH kinetics are most reliable. GLDH is the most specific laboratory marker with almost 90% specificity. The sensitivity of combination erythrocyte mean cell volume and GLDH kinetics after 1 week of abstinence is pathognomonic by 97.2%. GLDH is an equally accurate marker of alcoholism in comparison to others
glutamate dehydrogenase
diagnostics
GDH electrophoretic type (ETs) and sequence types may serve as useful markers in predicting the pathogenic behavior of strains of this serotype and that the molecular basis for the observed differences in the ETs is amino acid substitutions and not deletion, insertion, or processing uniqueness
phenylalanine dehydrogenase
diagnostics
the immobilized enzyme is useful for establishing an analytical spectrophotometric determination method of L-Phe in the diagnostics of phenyketonuria, an inborn error of amino acid metabolism in which the conversion of L-phenylalanine to L-tyrosine is impaired and can cause profound mental retardation if not detected and treated soon after birth. Early quantitative measurement of the plasma L-Phe is essential for the diagnosis of phenylketonuria and the control of dietary therapy of the patients. The conversion efficiency of the reactor with immobillized enzyme is 100% in the range of 0.005-0.6 mM Phe at 9 mM NAD+ with a total flow rate of 0.1 mL/min. The reactor is used for the analyses of 30 samples each for 3 h per day. The half-life period of the reactor is 15 days, method evaluation, overview
D-amino-acid oxidase
diagnostics
th enzyme is useful for measurement of D-serine contents in the brain via an implantable D-serine biosensor for in vivo monitoring
putrescine oxidase
diagnostics
enzyme can be used as biosensor for putrescine using dispersed multiwalled carbon nanotubes as ultra-microelectrodes ensuring a good electron transfer from the enzyme, method optimization, overview
protein-lysine 6-oxidase
diagnostics
LOX can serve as a predictive marker of lymph node metastasis and prognosis in esophageal squamous cell carcinoma. Overall survival rates of the patients with esophageal squamous cell carcinoma with high LOX expression are significantly lower than those of the patients with esophageal squamous cell carcinoma with low LOX expression
diamine oxidase
diagnostics
clinical impact of a commercial radioimmunoassay for determination of DAO activity for the diagnosis of histamine intolerance is evaluated in a prospective, multicentre study in 207 adult patients. No correlation between diamine oxidase serum levels and clinical status is be found
alanopine dehydrogenase
diagnostics
the significant positive relationship between exposure length and enzyme activity of ADH and strombine dehydrogenase, SDH, in Montipora capitata supports the implementation of these enzymes as biomarkers for rapid analysis of hypoxia-induced stress in corals
Strombine dehydrogenase
diagnostics
the significant positive relationship between exposure length and enzyme activity of alanopine dehydrogenase, ADH, and SDH in Montipora capitata supports the implementation of these enzymes as biomarkers for rapid analysis of hypoxia-induced stress in corals
sarcosine oxidase
diagnostics
developement of a simple and low cost electrochemical enzymatic biosensor for the determination of sarcosine in urine, based on the covalent immobilization of sarcosine oxidase, using N-ethyl-N-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide, on the surface of the screen-printed carbon electrode.The biosensor presents high analytical performance features, such as large concentration linear range (10-100 nM), low detection limit (16 nM) and large storage stability (60 days). The biosensor is successfully applied to the analysis of sarcosine in synthetic urine samples
sarcosine oxidase
diagnostics
a photobioelectrochemical sensor based on CdSe/ZnS quantum dot and sarcosine oxidase is developed to determine sarcosine concentrations. The linear range is from 0.1 mM to 1 mM and the sensitivity depends on the number of immobilised enzyme layers.It is demonstrated that the [SOD/PAH]6-layer system is well suited for sensitive sarcosine detection. Sarcosine is an intermediate in the enzymatic analysis of creatinine, and thus, a sarcosine sensor can be beneficial for the diagnosis of kidney diseases
sarcosine oxidase
diagnostics
sarcosine oxidase is an important diagnostic enzyme in the renal function examination
dimethylglycine dehydrogenase
diagnostics
the enzyme may be a valuable biomarker of hepatocellular carcinoma diagnosis
cytochrome-b5 reductase
diagnostics
potential use of the enzyme in diagnostic areas
hydrazine synthase
diagnostics
gene hzsA, encoding hydrazine synthase, is used as a biomarker for differentiation of biofilms and suspension, lab scale nitritation-anammox reactor with Kaldnes K1 carriers. Discrimination between Candidatus Brocadia fulgida and Candidatus Kuenenia stuttgartiensis based on hydrazine synthase subunit A (hzsA)
dihydrolipoyl dehydrogenase
diagnostics
Bacillus sphaericus DLD-diaphorase exhibits considerable potential to be used as a component of diagnostic tests for the quantification of metabolites
thiol oxidase
diagnostics
the expression of QSOX1 in neuroblastoma tumors may influence its clinical course because this protein is involved in processes such as the maturation of the extracellular matrix and the induction of apoptosis in these tumors. The enzyme can be used as a prognostic biomarker to help better discriminate among risk groups
thiol oxidase
diagnostics
QSOX1 is a potential prognostic marker which may prove of use in the staging of breast tumours and the stratification of breast cancer patients
chloride peroxidase (vanadium-containing)
diagnostics
the observed bactericidal and virucidal activity of the alkalophilic P395D/L241V/T343A mutant of vanadium chloroperoxidase is an important step forward in the application of this robust enzyme as a component in disinfection formulations
homogentisate 1,2-dioxygenase
diagnostics
crude enzyme preparations can be used for a spectrophotometric method for routine, sensitive determination of homogentisate in human urine
4-hydroxyphenylpyruvate dioxygenase
diagnostics
the enzyme can be used for enzyme-based sensors for monitoring herbicides used in agriculture, i.e. mesotrione. Compared to the standard sensors, biosensors have assorted advantages, such as practicality, quick response, low cost, and high sensitivity. A nanobiosensor is developed based on HPPD for mesotrione detection
beta-carotene 15,15'-dioxygenase
diagnostics
genotyping AI rams for c.196C-T can be used in selection against the yellow fat trait
Renilla-type luciferase
diagnostics
the split Renilla luciferase complementation assay (SRLCA) is one of the techniques that detect protein-protein interactions. The SRLCA is based on the complementation of the LN and LC non-functional halves of Renilla luciferase fused to possibly interacting proteins which after interaction form a functional enzyme and emit luminescence. The SRLCA can specifically detect the BGLF4/Hsp90 interaction and provide a reference to develop inhibitors that disrupt the Epstein-Barr virus kinase BGLF4 and heat shock protein Hsp90 interaction
Renilla-type luciferase
diagnostics
Renilla Luciferase (RLuc) is a blue light emitter protein which can be applied as a valuable tool in medical diagnosis
Cypridina-luciferin 2-monooxygenase
diagnostics
labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enyzme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2
Cypridina-luciferin 2-monooxygenase
diagnostics
Cypridina luciferase is used as bioluminescence reporter enzyme in yeast, bacterial, and mammalian cell-based assays, enzyme activity is measured with a luminometer by addition of native or synthetic luciferin, also known as Vargulin, 0.5 microM in 10 mM Tris-HCl, pH 7.4
Cypridina-luciferin 2-monooxygenase
diagnostics
far-red luminescence imaging technology to visualize tumor specific antigens on cell surfaces in vitro (human hepato-cellular carcinoma cell line Huh-7) and in living bodies (mice xenografted with human Delta-like protein-positive Huh-7 cells), based on a far-red fluorescent indocyanine derivative conjugated to biotinylated Cypridina luciferase linked to an anti-human Delta-like protein (Dlk-1) monoclonal antibody (embryonic antigens expressed on the surface of many cancer cells) via biotin-avidin interaction
inositol oxygenase
diagnostics
the kidney-specific protein myo-inositol oxygenase is a potential biomarker of acute kidney injury, AKI
[histone-H3]-lysine-9-demethylase
diagnostics
dimethylation of histone H3 at lysine 4 in combination with trimethylation of histone H3 at lysine 4 is associated with an activated transcriptional state. An increase of H3K4diMe in neoplastic tissues is predictive of clinical outcome. In particular, tumor recurrence occurs earlier in low-grade prostate carcinoma patients with low H3K4diMe, independently of other clinical and pathologic parameters. Similarly, in large cell and squamous cell carcinomas, low H3K4diMe expression correlates with short survival
[histone-H3]-lysine-9-demethylase
diagnostics
KDM4C pathway correlates with adverse pathologic phenotypes and poor overall survival in esophageal squamous cell carcinoma patients. Patients with co-expression of KDM4C and SOX2 have a poorer prognosis
procollagen-proline 4-dioxygenase
diagnostics
activation of NF-kappaB is observed in colon cancer. PHD3 appears to be a tumor suppressor in colorectal cancer cells that inhibits IKKbeta/NF-kappaB signaling. Determination of PHD3 status could aid targeted therapy selection for patients with colorectal tumors that have increased NF-kappaB activity
procollagen-proline 4-dioxygenase
diagnostics
the prolyl-4-hydroxylase alpha subunit 2 is a biomarker for breast cancer progression. Increased mRNA levels of P4HA2 correlate with poor clinical outcome in breast cancer patients, which is independent of estrogen receptor status
procollagen-lysine 5-dioxygenase
diagnostics
PLOD expression is associated with human breast cancer progression, PLOD2 expression is specifically prognostic in breast cancer
phytanoyl-CoA dioxygenase
diagnostics
the substrate phytanoyl-CoA is difficult to handle because of its amphipathic properties. Commercially available alternative substrates, such as hexadecanoyl-CoA or isovaleryl-CoA are potentially usefull
phytanoyl-CoA dioxygenase
diagnostics
assays for Refsum‘s disease should not be based on PAHX activity alone. At least four different types of mutation cause loss of PAHX activity in vivo. Mutations to the peroxisomal targeting sequence do not affect catalytic function but probably affect targeting or degradation of the enzyme. A second group of mutations, including truncation and missense mutations, results in total loss of activity. A third group of mutations results in uncoupling of substrate oxidation from that of 2-oxoglutarate. A fourth group of mutations causes partial inactivation by hindering binding/utilization of the 2-oxoglutarate cosubstrate and/or iron(II) cofactor. From the therapeutic and biochemical view-point the latter two groups are particularly interesting since it may be possible to restore their activity with modified cosubstrates
salicylate 1-monooxygenase
diagnostics
usage of the enzyme in a fluorescence assay that detects 3-hydroxybutyrate and cholesterol in the nanomolar range and is more sensitive than the current SHL-dehydrogenase amperometric sensors, making it applicable to the construction of a fiber-optic fluorescence biosensor for clinical diagnostic uses
bacterial luciferase
diagnostics
expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview; expression of the bacterial luciferase system in mammalian cells for generation of bioreporters for in vivo monitoring and diagnostics technology, method evaluation and optimization, overview
tyrosine 3-monooxygenase
diagnostics
tyrosine hydroxylase is frequently used as a marker of dopaminergic neuronal loss in animal models of Parkinson’s disease
dopamine beta-monooxygenase
diagnostics
genotype-controlled measurement of plasma DBH activity might be used as a potential biological marker of the response to trauma
acyl-CoA 6-desaturase
diagnostics
higher delta-6-desaturase activity is associated with a higher risk of developing metabolic syndrome
Results 1 - 100 of 479 > >>