1.6.2.4: NADPH-hemoprotein reductase

This is an abbreviated version!
For detailed information about NADPH-hemoprotein reductase, go to the full flat file.

Word Map on EC 1.6.2.4

Reaction

NADPH
+
H+
+ n oxidized hemoprotein =
NADP+
+ n reduced hemoprotein

Synonyms

aldehyde reductase (NADPH-dependent), ATR2, CPR, CPR I, CPR-X1, CPR-X2, CPR1, CPR2, CYPOR, cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent), cytochrome P450 NADPH reductase, cytochrome P450 oxidoreductase, cytochrome P450 reductase, cytochrome P450-diflavin reductase, dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase, EC 1.6.99.2, FAD-cytochrome c reductase, ferrihemprotein P450 reductase, flavocytochrome P450 BM3, NAD(P)H cytochrome P450 reductase, NADP-cytochrome c reductase, NADP-cytochrome P-450 reductase, NADP-cytochrome reductase, NADPH cytochrome P450 reductase, NADPH dependent cytochrome P450 reductase, NADPH P450 oxidoreductase, NADPH P450 reductase, NADPH-CPR, NADPH-CYP reductase, NADPH-CYP450 reductase, NADPH-cytochrome c oxidoreductase, NADPH-cytochrome c reductase, NADPH-cytochrome p-450 reductase, NADPH-cytochrome P450 (CYP) oxidoreductase, NADPH-cytochrome P450 oxidoreductase, NADPH-cytochrome P450 reductase, NADPH-cytochrome P450 reductase 2, NADPH-dependent cytochrome c reductase, NADPH-dependent cytochrome P450 reductase, NADPH-dependent P450 reductase, NADPH-ferricytochrome c oxidoreductase, NADPH-ferrihemoprotein reductase, NADPH-P450 reductase, NADPH: cytochrome P450 oxidoreductase, NADPH: cytochrome P450 reductase, NADPH:CYP oxidoreductase, NADPH:cytochrome P450 oxidoreductase, NADPH:cytochrome P450 reductase, NADPH:ferrihemoprotein oxidoreductase, neotetrazolin reductase, NPR, P-450 reductase, P450 BM3, P450 oxidoreductase, P450 Red, P450 reductase, P450R, P450REd, POR, RedA, reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase, reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate), SbCPR2, TcCPR-A, TcCPR-B, TcCPR-C, TPNH-cytochrome c reductase, TPNH2 cytochrome c reductase, WsCPR1, WsCPR2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase

Cloned

Cloned on EC 1.6.2.4 - NADPH-hemoprotein reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
; expressed in Escherichia coli strain TOP10 and coexpressed with CYP98A14 in Saccharomyces cerevisiae strain INV Sc1
canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid
chimeric protein with His-tag
Saccharomyces cerevisiae /Homo sapiens
coexpressed together with a form of cytochrome P450 in Salmonella typhimurium TA1538
-
coexpression with CYP3A4 in Spodoptera frugiperda and Trichoplusia ni cell lines
-
coexpression with two differently modified CYP2D6 cDNAs in Escherichia coli, 17alpha-2D6 + pJR7 and ompA-2D6 + pJR7
-
DNA and amino acid sequence determination and analysis, sequence comparisons, the enzyme shows highly conserved exon/intron organization structure, functional expression in Escherichia coli membranes
expressed in Bacillus subtilis DB104 under the transcriptional control of an IPTG-inducible fusion promoter of PgroE and Ptac. The expressed rCPR is released into the culture medium after sporulation by autolysis of the host cell. It is associated with and displayed on the spore surfaces
-
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21 Star cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) pLysS cells
-
expressed in Escherichia coli C41 cells
-
expressed in Escherichia coli C43(DE3) cells
-
expressed in Escherichia coli codon plus cells
-
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli in Sf9 insect cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli XL-1 Blue cells
-
expressed in Escherichia coli XL1-Blue cells
expressed in HeLa cells
-
expressed in microsome from baculovirus-infected insect cells
-
expressed using a pET/Escherichia coli system
expression in Escherichia coli
expression in Escherichia coli; expression in Escherichia coli; expression in Escherichia coli
expression in Saccharomyces cerevisiae
-
expression in transformed strains
-
expression in transformed strains of Saccharomyces cerevisiae
-
expression of CPR2 using the modified pYeDP60 vector produces high amounts of active protein, expression in Saccharomyces cerevisiae strain W303
-
expression of missense variant proteins lacking 27 N-terminal residues in Escherichia coli
-
functional expression of mammalian NADPH-cytochrome P450 oxidoreductase on the cell surface of Escherichia coli
-
gene 147CC514, expression of wild-type, truncated mutant, and point mutations in Escherichia coli strain C41(DE3)
gene cpr, DNA and amino acid sequence determination and analysis, expression of the N-terminally truncated and His6-tagged enzyme, lacking the putative membrane anchor, in Escherichia coli strain BL21 (DE3)
gene CsCPR, real-time quantitative PCR expression analysis and phylogenetic tree, heterologous expression of N-terminally truncated and His6-tagged protein in Escherichia coli Rosetta (DE3) cells
gene encoding CPr, DNA and amino acid sequence determination and analysis, expression pattern and phylogenetic analysis
-
isoform CPR1 is expressed in Escherichia coli Rosetta(DE3)pLysS cells
NADPH-cytochrome c oxidoreductase lacking the first 55 amino acid residues (DELTA55AnCY) is expressed in Escherichia coli loses FMN, leading to an unstable protein and subsequent aggregation. The triple mutant L86F/L219F/P456FDELTA55AnCY exhibits increased stability with significant reduction in aggregation compared to the wild-type enzyme
-
native and truncated form expressed in Escherichia coli BL21(DE3)pLysS as His tag fusion protein
-
recombinant enzyme expression in Escherichia coli strain DH5alpha
recombinant expression in Escherichia coli
-
the complete CPR cDNA is expressed as 6*His-tagged fusion protein in both membrane and cytosolic fractions in Escherichia coli. Both fractions contain NADPH-cytochrome c reducing activity. The membrane-bound form containing N-terminal membrane anchor is subjected to purification
to analyze the molecular interaction among CYP3A4, NADPH-cytochrome P450 oxidoreductase, and b5, several fused enzyme genes are constructed and expressed in Saccharomyces cerevisiae. The recombinant fused enzymes CYP3A4-truncated(t)-P450 reductase-t-b5 (3RB) and CYP3A4-t-b5-t-P450 reductase (3BR) in yeast microsomes shows a higher specific activity in 6beta-hydroxylation of testosterone than does the reconstitution premixes of CYP3A4, P450 reductase, and b5. The purified fused enzymes exhibit lower Km values and substantially increased Vmax values in 6beta-hydroxylation of testosterone and oxidation of nifedipine. The fused enzymes shows significantly higher activities in cytochrome c reduction than the reconstitution premixes. Although the affinity of 3RB toward cytochrome c is twice as high as that of 3BR, 3BR and 3RB show nearly the same affinity toward NADPH/NADH. In addition, the heme of the CYP3A4 moiety of 3RB is reduced preferentially and more rapidly than that of 3BR, whereas the heme of the b5 moiety of 3BR is selectively reduced compared with that of 3RB
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truncated form lacking the membrane anchoring domain expresed in Escherichia coli BL21(DE3)pLysS
-
two different domains separately expressed in Escherichia coli BL21(DE3)
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variants of Depressaria pastinacella CYP6AB3v2 are co-expressed with P450 reductase from Musca domestica in Sf9 cells. Allelic variation in the Depressaria pastinacella CYP6AB3 protein enhances metabolism of plant allelochemicals by altering a proximal surface residue and potential interactions with cytochrome P450 reductase
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wild-type and mutant K56Q, expression in Escherichia coli
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WsCPR1, phylogenetic analysis and quantitative real-time PCR expression analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3); WsCPR2, phylogenetic analysis and quantitative real-time PCR expression analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 (DE3)