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1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

anthranilate
+
N,N-dimethyl-1,4-phenylenediamine
 + 2 NAD+ =
2-(4-dimethylaminophenyl)diazenylbenzoate
 + 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.17 FMN-dependent NADH-azoreductase

Specific Activity

Specific Activity on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.626
-
cofactor NADPH, pH 7.0, 25°C
11.4
substrate orange I, pH 7.4, 30°C
137
substrate amaranth, pH 7.1, 23°C
139
substrate orange II, pH 7.1, 23°C
141.25
purified recombinant enzyme, pH 4.0, 25°C
167
substrate ponceau S, pH 7.1, 23°C
18
-
substrate orange G, cofactor NADH, pH 7.4, 37°C
181
substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid, pH 7.1, 23°C
188.6
purified recombinant enzyme, pH 7.4, 30°C, methyl red with FMN
22
-
substrate amaranth, cofactor NADPH, pH 7.4, 37°C
24.1
substrate 1-(2-pyridylazo)-2-naphthol,pH 7.4, 30°C
25
-
substrate amaranth, cofactor FADH2, pH 7.4, 37°C
25.8
30°C, pH 7.4, substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid
26
-
substrate amaranth, cofactor FMNH2, pH 7.4, 37°C
26.6
substrate 4'-(dimethylamino)-azobenzene-2-carboxylic acid, pH 7.4, 30°C
362.6
purified recombinant enzyme, pH 7.4, 30°C, menadione with FMN
441
purified recombinant enzyme, pH 7.4, 30°C, ethyl red with FMN
63
-
substrate fast red E, cofactor NADH, pH 7.4, 37°C
63.9
pH 7.4, 30°C
7.3
substrate acid red 88, pH 7.4, 30°C
74
-
substrate amaranth, cofactor NADH, pH 7.4, 37°C
98.9
purified recombinant enzyme, pH 7.4, 30°C, methyl red with FAD