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1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

anthranilate
+
N,N-dimethyl-1,4-phenylenediamine
 + 2 NAD+ =
2-(4-dimethylaminophenyl)diazenylbenzoate
 + 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.17 FMN-dependent NADH-azoreductase

Cloned

Cloned on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning and sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
gene acpD, sequence comparisons, recombinant overexpression of the gene provides Escherichia coli strain JM109 with more than 800 times increased azoreductase activity
gene azoR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene azoR, recombinant expression of His-tagged wild-type and mutant AzoR enzymes in Escherichia coli BL21(DE3)
gene azoR, recombinant overexpression of His-tagged enzyme in Escherichia coli strain XL1-Blue