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2.5.1.18: glutathione transferase

This is an abbreviated version!
For detailed information about glutathione transferase, go to the full flat file.

Word Map on EC 2.5.1.18

Reaction

RX
+
glutathione
=
HX
 +
R-S-glutathione

Synonyms

2-hydroxychromene-2-carboxylic acid isomerase, 26GST, adGSTD4-4, agGSTe2, allergen Der p 2, allergen Der p 8, alpha class glutathione transferase, Alpha class GST, alpha GST, alpha-class glutathione transferase, asGST5.5, AtGSTF2, AtuGSTH1-1, BaeAB, beta-etherase, bmGSTD, BphK, CgGSTM1, CgGSTM2, cGST, Chi class GST, Chi GST, class mu glutathione S-transferase, delta class glutathione transferase, delta-class glutathione S-transferase, Delta-class glutathione transferase, EC 1.8.6.1, EC 2.5.1.12, EC 2.5.1.13, EC 2.5.1.14, EC 4.4.1.7, EGST, epsilon class GST, Epsilon glutathione S-transferase, Epsilon glutathione transferase, Epsilon GST, epsilon-class glutathione S-transferase, epsilon-class glutathione transferase, GHR, glutathione S-alkyl transferase, glutathione S-aralkyltransferase, glutathione S-aryltransferase, glutathione S-transferase, glutathione S-transferase 2, glutathione S-transferase A1-1, glutathione S-transferase A3-3, glutathione S-transferase AdFSTD3-3, glutathione S-transferase I, glutathione S-transferase omega 1, glutathione S-transferase omega 2, glutathione S-transferase P1-1, glutathione S-transferase pi, glutathione S-transferase Pi-1, glutathione S-transferase X, glutathione transferase, glutathione transferase A1-1, glutathione transferase A4-4, glutathione transferase M1-1, glutathione transferase M2-2, glutathione transferase Omega 3S, glutathione transferase omega-1, glutathione transferase P1-1, glutathione transferase Pi, glutathione transferase T1-1, glutathione transferase zeta, glutathione transferase zeta 1, glutathione transferase Zeta 1-1, glutathione transferase zeta1-1, glutathione transferase-like protein, glutathione-S-transferas, glutathione-S-transferase, glutathione-S-transferase pi, glutathione-transferase, GmGSTU4-4, GSH S-transferase, GSH transferase, GSH transferase homologue, GSH-S transferase rho, GSHTase-P, GST, GST A1-1, GST A2-2, GST A3-3, GST A4-4, GST adgstD4-4, GST alpha, GST Delta 2, GST I, GST II, GST III, GST IV, GST M1-1, GST M2-2, GST M4-4, GST M5-5, GST mu, GST O1-1, GST P1-1, GST pi, GST T1-1, GST Tau, GST Tau19, GST Z1-1, GST-1, GST-2, GST-26, GST-3, GST-Acr, GST-OCX-32, GST-T, GST-theta, GST1, GST1b, GST2, GST20, GST3, GST4, GST5, GST5118, GST7, GST83044, Gsta, GSTA1, GSTA1-1, GSTA2-2, GSTA3, GSTA3-3, GSTA4, GSTA4-4, GSTA4L, GSTA5, GSTA5-5, GSTalpha1, GSTD, GSTD1, GSTD10, GSTd14, GSTD2, GSTD4, GSTD4-4, GSTE1, GSTE2, GSTE3, GSTE4, GSTE5, GSTE6, GSTE7, GSTE8, GSTF12-1, GSTF12-2, GSTF3, GSTF5, GSTF9, GSTFuA1, GSTk, GSTK1, GSTL1, GSTL2, GSTL3, Gstm, GSTm09, GSTM1, GSTM1-1, GSTM2, GSTM2-2, GSTM3, GSTM3-3, GSTM4, GSTM4-4, GSTM5, GSTM5-5, GSTmu, GSTO, GSTO1, GSTO1-1, GSTO2, GSTO2-2, GstO2A, GstO2B, GSTO3, GSTO3S, GSTO4, GSTO7, GSTP, GSTP 1-1, GSTP-1, GSTP1, GSTP1-1, Gstr1, GSTrho, GSTS, GSTS1, GSTS1-1, GSTS2, GSTS3, GSTT, GSTT1, GSTT1-1, Gstt1a, GSTT2, GSTT2-2, GSTT2B-2B, GSTT4, GSTT4L, GSTU1, GSTU10, GSTU10-10, GSTU11, GSTU12, GSTU13, GSTU13-1, GSTU14, GSTU16, GSTU17, GSTU18, GSTU19, GSTU2, GSTU2-2, GSTU21, GSTU24, GSTU24-1, GSTU25, GSTU26, GSTU28, GSTU3, GSTU4, GSTU45, GSTU5, GSTU6, GSTU7, GSTU8, GSTU9, GSTZ, Gstz1, GSTZ1-1, GTT1.2, HCCA isomerase, Hematopoietic prostaglandin D synthase, hGSTA-3, hGSTA1-1, hGSTZ1-1, kappa class glutathione transferase, kappa class GSH transferase, Kappa class GST, KKSG9, lambda glutathione transferase, MAAI, MGST1, MGST2, MGST3, Mgst3a, Mgst3b, microsomal glutathione transferase 1, microsomal glutathione transferase-1, More, mtMGST1, mu class glutathione S-transferase, Mu class GST, mu glutathione transferase, Mu GST, mu-class glutathione S-transferase, mu-class glutathione S-transferase1, Mu-class GST, Mu-GST, nu-class glutathione transferase, Omega class GST, omega glutathione S-transferase, omega glutathione transferase, omega-class glutathione S-transferase, omega-class glutathione S-transferase 2, pGSTA1, phi class glutathione transferase, Pi class GST, pm-GSTR1, PmGST, PtGSTU1, PvGSTF1-1, PvGSTU2-2, PvGSTU3-3, rho class glutathione S-transferase, rho-GST, RX: glutathione R-transferase, RX:glutathione R-transferase, S-(hydroxyalkyl)glutathione lyase, Saro_2873/Saro_2872, selenium-containing glutathione transferase zeta1-1, seleno-hGSTZ1-1, SGST26.5, sigma class glutathione transferase, Sigma class GST, sigma glutathione S-transferase, sigma-class glutathione S-transferase, sigma-class GST, SIGST, Sj26GST, sjGST, sll1545, ssGST3, ssGST5, tau class glutathione S-transferase, tau class glutathione transferase, tau class GST, tau class GSTU4-4, TDR1, theta class glutathione S-transferase, theta class glutathione transferase T1-1, theta class GST, theta-class glutathione transferase, thiol-dependent reductase I, Ure2p mutant A122C, Ure2pB1, Xi class glutathione transferase, Ya-GST, YghU, zeta class glutathione S-transferase, Zeta class GST

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.18 glutathione transferase

Purification

Purification on EC 2.5.1.18 - glutathione transferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1,4-butanediol diglycidyl ether-GSH-Sepharose-CL6B column chromatography
13 isoforms
-
2 forms: GST I, GST II
-
2 isozymes
-
200fold, isoenzyme A, B and C
-
3 forms, homogeneity
-
5 major: I, IV, V, VI, VII and 3 minor forms
-
7 major isoenzymes
-
affinity column chromatography
-
ammonium sulfate precipitation, carboxymethyl-Sepharose column chromatography, and GSH-Sepharose affinity chromatography followed by ultrafiltration
-
ammonium sulfate precipitation, DEAE cellulose column chromatography, and ACA44 gel filtration
-
ammonium sulfate precipitation, Q Sepharose column chromatography, GSTrap column chromatography, and High Q column chromatography
-
amylose resin column chromatography
an acidic and a basic form
-
anion exchange chromatography and Superdex S200 gel filtration
basic and acidic isoenzymes
-
DEAE column chromatography and glutathione-affinity Sepharose column chromatography
DEAE Sepharose column chromatography and nickel affinity chromatography
DEAE-Sepharose column chromatography and GSH-Sepharose column chromatography
-
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
forms: alpha, beta, gamma, delta, epsilon and a species with a very low isoelectric point
-
four isoenzymes
-
glutathione affinity chromatography
glutathione agarose affinity chromatography
-
glutathione agarose affinity column chromatography
-
glutathione agarose column chromatography
-
glutathione Seharose 4B column chromatography
glutathione Sepharose column chromatography
glutathione-agarose affinity chromatography and carboxymethyl Sepharose column chromatography
-
glutathione-agarose affinity column chromatography
-
glutathione-Sepharose 6B column chromatography
-
glutathione-Sepharose column chromatography
GSH affinity column chromatography and Superdex 75 gel filtration
GSH-affinity column chromatography
-
GSH-agarose affnity column chromatography, DEAE-Sephadex gel filtration, and GSH-agarose affinity column chromatography
-
GSH-agarose bead chromatography and Superdex 200 gel filtration
GSH-agarose column chromatography and Superdex S200 gel filtration
Coturnix sp.
-
GST affinity column chromatography
-
GST pi
-
GST Trap column chromatography
GSTC: activity towards 1-chloro-2,4-dinitrobenzene, GSTF: activity towards the herbicide fluorodifen, GPOX: peroxidase activity
-
GSTrap 4B column chromatography, HiTrap Q column chromatography, and DEAE-Sepharose column chromatography
GSTrap affinity column chromatography
GSTrap column chromatography
GSTrap-Hp column chromatography
hexyl-glutathione-agarose bead chromatography
His-Trap column chromatography and Superdex 200 gel filtration
His60 Ni Superflow resin column chromatography and Superdex 200 gel filtration
homogeneity
homogeneity, 2 isoenzymes: GST I-I and GST II-III
-
homogeneity, 4 enzyme fractions
-
homogeneity, anionic enzyme
-
IDA-Ni2+ affinity column chromatography and glutathione Sepharose 4B column chromatography
IMAC Sepharose 6 column chromatography
-
isoenzyme 7-7
-
isoenzyme A and C
-
isoenzyme A, AA, B, C and E
-
isoenzyme P1-1
-
isozyme GSH-S transferase rho 340fold from erythrocytes by acetone precipitation, ethanol-chloroform treatment, two different steps of anion exchange chromatography, and gel filtration
-
maltose-affinity amylose column chromatography
most abundant inducible GST isoenzyme after treatment with safener
-
multiple forms
-
native alpha class isozymes GSTA2-2, GSTA1-2 and GSTA1-1 from kidney by anion exchange and glutathione affinity chromatography, followed by another step of ion exchange chromatography
-
native cytosolic isozyme 220fold from digestive glands by ultracentrifugation, gel filtration, and glutathione affinity chromatography
native cytosolic liver GST 97fold by glutathione affinity chromatography
native enzyme 106fold from gills by glutathione affinity chromatography to homogeneity
-
native enzyme 9.9fold by subcellular fractionation, ammonium sulfate fractionation, glutathione affinity chromatography, and ultrafiltration
-
native enzyme by anion exchange and glutathione affinity chromatography
-
native enzyme by glutathione affinity and anion exchange chromatography
native enzyme by glutathione affinity chromatography
-
native enzyme by glutathione affinity chromatography to homogeneity
native enzyme by glutathione affinity chromatography, three field populations from Nanyang, purification 42.5fold, Wuzhou, purification 64.5fold, and Hezhou purification 67.4fold
-
native enzyme partially by mitochondrion isolation
-
native enzyme partially by subcellular fractionation
-
native enzyme partially from shoots by ammonium sulfate fractionation and desalting
native fluazifop-p-butyl-inducible GSTs PvGSTF1-1, PvGSTU2-2 and PvGSTU3-3 from leaves by glutathione affinity chromatography and dialysis
-
native isozyme SIGST 19fold to homogeneity by ammonium sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration
native isozymes by affinity chromatography and gel filtration or electrophoresis
native isozymes GST2 and GST3 by ion exchange and glutathione affinity chromatography to homogeneity
-
native Sigma and Delta class isozymes by gel filtration, glutathione affinity chromatography, and anion exchange chromatography, 103fold from pupae, 100fold from larvae, 51.7fold from egg (only Delta GST isozyme), and 52fold from adult
near homogeneity, 2,4-dichloro-phenoxyacetic acid-inducible GST isoenzyme
-
Ni+-NTA column chromatography
Ni-IMAC affinity column chromatography
Ni-NTA affinity column chromatography
-
Ni-NTA agarose chromatography
-
Ni-NTA agarose column chromatography, and gel filtration
-
Ni-NTA agarose resin column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography and GSH-Sepharose column chromatography
-
Ni-NTA column chromatography, and gel filtration
-
Ni-NTA Sefinose resin column chromatography
-
Ni-Sepharose column chromatography
Ni-Sepharose column chromatography, and gel filtration
Ni2+-affinity chromatography and Superdex S200 gel filtration
-
Ni2+-affinity column chromatography and Superdex S200 gel filtration
-
Ni2+-affinity resin column chromatography
Ni2+-NTA agarose column chromatography
nickel affinity resin chromatography
nickel affinity resin column chromatography
nickel-affinity column chromatography
-
nickel-agarose column chromatography
nickel-chelating column chromatography
-
nickel-immobilized metal ion affinity chromatography
omega isoenzyme
P1-1, A1-1 and M1-1
-
Q-Sepharose column chromatography and GSH-agarose affinity chromatography
-
recombinant
recombinant BphK from Escherichia coli strain GJ1158 by glutathione affinity chromatography
-
recombinant cytosolic isozyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
-
recombinant Der p 8 from Pichia pastoris by glutathione affinity chromatography, native enzyme Der p 2 by glutathione affinity chromatography
-
recombinant enzyme
recombinant enzyme by glutathione affinity chromatography
-
recombinant enzyme form GSTR1
recombinant enzyme from Escherichia coli by glutathione affinity chromatography and dialysis
recombinant enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to over 95% purity
-
recombinant enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography to homogeneity
recombinant enzyme from Escherichia coli to homogeneity
recombinant GST adgstD4-4 mutant V107A from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
-
recombinant GST2
-
recombinant GSTD1 and GSTD10 from Escherichia coli strain BL21(DE3)pLysS by anion exchange and hydrophobic interaction chromatography
-
recombinant GSTP1-1 from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis
-
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, recombinant enzyme from Escherichia coli strain DH5alpha by gel filtration
recombinant His-tagged GST-1 from Pichia pastoris strain X-33 by nickel affinity chromatography
recombinant His-tagged isozyme GSTO2 by glutathione affinity chromatography
recombinant His-tagged wild-type and mutant AtuGSTH1-1s from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL1-Blue by nickel affinity chromatography
-
recombinant His-tagged wild-type enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography
-
recombinant His6-tagged wild-type hGSTZ1-1 and mutant seleno-hGSTZ1-1 from HEK-293T cells by nickel affinity chromatography
-
recombinant Ile2 enzyme variant from Escherichia coli strain BL21 by glutathione affinity chromatography
recombinant isozyme GST A1-1 from Escherichia coli by ammonium sulfate fractionation, gel filtration, S-hexylglutathione affinity chromatography, ultrafiltration, and again gel filtration
recombinant isozyme GSTA1-1 from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography
-
recombinant isozyme GSTA1-1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, recombinant His-tagged isozymes GSTO1-1 and GSTT2-2 in Escherichia coli strain M15[pREP4] by nickel affinity chromatography
-
recombinant isozyme GSTO from Escherichia coli to homogeneity by ammonium sulfate fractionation, anion exchange chromatography and gel filtration
recombinant isozyme GSTT1 from Escherichia coli
recombinant isozymes from Escherichia coli by S-hexylglutathione affinity chromatography to homogeneity
-
recombinant isozymes GST A1-1 and GST A4-4 and recombinant mutant GIMFhelix from Escherichia coli by glutathione affinity chromatography
-
recombinant isozymes GSTP1-1 and GSTM2-2 from Escherichia coli
-
recombinant isozymes SoGST3 and SoGST6 from Escherichia coli strain BL21 (DE3) by anion exchange chromatography, ammonium sulfate fractionation, cation exchange chromatography, and again two steps of anion exchange chromatography on different resins
-
recombinant N-terminally His6-tagged Epsilon GSTs from Escherichia cli by nickel affinity chromatography and gel filtration
-
recombinant N-terminally His6-tagged isozyme MdGST6B from Escherichia coli strain BL21 CodonPlus (DE3)-RIL by nickel affinity chromatography
recombinant N-terminally Strep-tagged AtGSTF2 and AtGSTF3 from Escherichia coli by affinity chromatography and gel filtration
-
recombinant protein after expression in E. coli
-
recombinant soluble enzyme from supernatant by anion exchange chromatograpy and gel filtration
-
recombinant wild-type and mutannt isozymes GSTU4-4 by glutathione affinity chromatography
recombinant wild-type and mutant bmGST from Escherichia coli strain Rosetta (DE3)pLysS by anion exchange chromatography and gel filtration
-
recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange chromatography and S-hexylglutathione affinity chromatography, respectively
-
recombinant wild-type and mutant enzymes from Escherichia coli strain XL1-Blue by anion exchange chromatography, dialysis, ultrafiltration, and chromatofocussing
-
recombinant wild-type and mutant GST5118 from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, followed by dialysis and ultrafiltration
recombinant wild-type and mutant GSTP1-1s from Escherichia coli by glutathione affinity chromatography
recombinant wild-type and mutant isozyme GSTA4-4, and recombinant isozyme GSTA1-1GIMFhelix mutant from Escherichia coli by glutathione affinity chromatography and gel filtration
-
recombinant wild-type and mutant N-terminally His6-tagged isozymes GST T1-1 from Escherichia coli strain XL-1 blue by nickel affinity chromatography
-
recombinant wild-type and mutant R15L isozyme GSTA1-1 from Escherichia coli strain BL21(DE3) by ion exchange chromatography
recombinant YghU from strain BL21(DE3) by anion and cation exchange chromatography and dialysis
-
S-hexylglutathione-affinity column chromatography and concanavalin A-agarose column chromatography
-
Sepharose-glutathione column chromatography
Strep-Tactin Sepharose column chromatography and Superdex S200 gel filtration
transferase 1, 2.1 and 2.2
-
wild type and mutants
-